I aggregated every rt-PCR primer published into a single database

Name: I aggregated every rt-PCR primer published into a single database
Availability: InStock
Author: tmolley

by tmolley·Jun 2, 2026·3 points·0 comments

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AI Analysis

●●●BangerSolve My ProblemNiche GemZero to One

7M primers from 400K papers - why didn't this aggregated database exist before?

Strengths

•Nature Communications publication validates the research methodology and data quality
•Solves genuine pain point of failed primers for common genes like GAPDH and ACTB
•355K primer pairs across 31K genes with citation-based ranking for reliability

Weaknesses

•Not for clinical use limits potential applications to research settings only
•Niche audience means limited broader appeal outside molecular biology labs

Post Description

Just had our tool published in nature comms: https://www.nature.com/articles/s41467-026-73648-2

If anyone else has dealt with the pain of trying to make tons of PCR primers and have half of them fail, maybe you'll appreciate this!

We were inspired when one of my grad students bought a GAPDH primer that failed and I was like "Why the heck are we constantly remaking primers for such common genes"